5/7/2023 0 Comments Counter clockwise langer![]() ![]() Heart decellularization creates acellular myocardial scaffolds that perfectly mimic the native architecture, challenging to achieve with synthetic materials. The needle used for the cannulation was inserted less than 1 cm, not too low as it could lead to occlusion of coronary ostium or even to the destruction of aortic valves, compromising the experiment ( Figure 1a,b). The organ was attached to the Langendorff device using a 3-0 silk surgical suture. Further, the heart and the ascending aorta were removed from the chest and washed with heparinized saline solution. Before the heart explantation, the superior and inferior vena cava, the pulmonary veins, and the pulmonary artery were transected. Median sternotomy allowed access to the mediastinum. Subsequently, the inferior vena cava was exposed and, systemic heparinization was performed using unfractionated heparin (Pan Pharma GmbH, La Selle-en-Luitré, France) at 3.0 IU/g. Access to the retroperitoneum was gained by shifting aside the intestine package. Pilosity was removed from the entire abdominal–thoracic area and continued with the skin and muscular layer incision. ![]() ![]() Animals were accordingly prepared to allow sterile surgical access. The protocol included general anesthesia starting with induction at 8% and maintenance at 3.5%. All animals used in the generation of heart scaffolds were anesthetized with sevoflurane (AbbVie Deutschland GmbH & Co. The average weight heart dissected from the rats was 1.28 ± 0.12 g. The hearts had their weight measured on a precision scale (Intelligent-Lab™ HT-84 Analytical Balance). Heart extracellular matrices were derived from ten male Sprague Dawley rats of 250–350 g (12–16 weeks old). Optimizing the decellularization process using a machine learning approach launches exponential progress in tissue bioengineering research. A decellularization completion metric was built using a DCNN based classifier model trained using an image set comprising thousands of photos. This system aimed to prove a strong correlation between the data gathered by spectrophotometry and the state of the heart that could be visualized with an OpenCV-based spectrometer. A monitoring system supervised the sessions, collecting a large number of photos saved in corresponding folders. Spectrophotometric measurements of deoxyribonucleic acid (DNA) and total proteins concentration from the decellularization solution were taken every 30 min. Hearts from male Sprague Dawley rats (n = 10) were decellularized using 1% sodium dodecyl sulfate (SDS) in a modified Langendorff device in the presence of an alternating rectangular electric field. Our proposal is a software application using deep convolutional neural networks (DCNN) to distinguish between different stages of decellularization, determining the exact moment of completion. Unfortunately, there is no objective gold standard evaluation of the scaffold without applying a destructive method such as histological analysis or DNA removal quantification of the dry tissue. Counterclockwise is sure to become a standard source on new-century science and healing.Whole organ decellularization techniques have facilitated the fabrication of extracellular matrices (ECMs) for engineering new organs. A hopeful and groundbreaking book by an author who has changed how people all over the world think and feel. Immensely readable and truly fascinating, Counterclockwise offers a transformative and bold new paradigm: the psychology of possibility. With only subtle shifts in our thinking, our language, and in our expectations, she tells us that we can begin to change the ingrained behaviour that sap health, optimism, and vitality from our lives. ![]() Drawing on landmark work in the field and her own body of colourful and highly original experiments, Langer shows that the magic of rejuvenation and ongoing good health lies in being aware of the ways we mindlessly react to social and cultural cues. If we could turn back the clock psychologically, could we also turn it back physically? For more than thirty years, award-winning psychologist Ellen Langer has studied this provocative question, and now, in Counterclockwise, she presents a conclusive answer: opening our minds to what's possible, instead of clinging to notions about what's not, can lead to better health - at any age. ![]()
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